A genetic system for biasing the sex ratio in mice

Ido Yosef, Liat Edry-Botzer, Rea Globus, Inbar Shlomovitz, Ariel Munitz, Motti Gerlic, Udi Qimron

Research output: Contribution to journalArticlepeer-review

Abstract

Biasing the sex ratio of populations of different organisms, including plants, insects, crustacean, and fish, has been demonstrated by genetic and non-genetic approaches. However, biasing the sex ratio of mammalian populations has not been demonstrated genetically. Here, we provide a first proof of concept for such a genetic system in mammals by crossing two genetically engineered mouse lines. The maternal line encodes a functional Cas9 protein on an autosomal chromosome, whereas the paternal line encodes guide RNAs on the Y chromosome targeting vital mouse genes. After fertilization, the presence of both the Y-encoded guide RNAs from the paternal sperm and the Cas9 protein from the maternal egg targets the vital genes in males. We show that these genes are specifically targeted in males and that this breeding consequently self-destructs solely males. Our results pave the way for a genetic system that allows biased sex production of livestock.

Original languageEnglish
Article numbere48269
JournalEMBO Reports
Volume20
Issue number8
DOIs
StatePublished - 2019

Keywords

  • CRISPR-Cas
  • Y chromosome
  • genetic system
  • mammals
  • sex ratio

Fingerprint

Dive into the research topics of 'A genetic system for biasing the sex ratio in mice'. Together they form a unique fingerprint.

Cite this