A genetic sequence change in the 3'-noncoding region of the androgen receptor gene in prostate carcinoma

A. Paz, A. Lindner, A. Zisman, Y. Siegel

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: We intended to identify structural changes in the hormone-binding domain and C-terminal region of the androgen receptor (AR) gene in carcinoma of prostate specimens. Methods: Twelve prostate cancer specimens from prostate resections were analyzed for the presence of mutations in the E-H exons and part of the 3'-untranslated region of the AR cDNA, using RT-PCR and direct sequencing. Results: A polymorphic sequence spanning 17 nucleotides was identified in the 3'-untranslated region of the AR cDNA from one prostate carcinoma tissue, suggesting the presence of a somatic mutation. No mutations were identified in the coding sequence of the hormone-binding domain of the AR cDNA. Conclusion: These results suggest that AR mutations are probably uncommon molecular events in the early stages of prostate cancer.

Original languageEnglish
Pages (from-to)209-215
Number of pages7
JournalEuropean Urology
Volume31
Issue number2
DOIs
StatePublished - 1997

Keywords

  • Androgen receptor gene
  • Direct sequencing
  • Polymerase chain reaction
  • Prostate carcinoma
  • Somatic mutation

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