@article{0401cc7ffde7479188c7e638950789f7,
title = "A Functional Chemiluminescent Probe for in Vivo Imaging of Natural Killer Cell Activity Against Tumours",
abstract = "Natural killer (NK) cells are immune cells that can kill certain types of cancer cells. Adoptive transfer of NK cells represents a promising immunotherapy for malignant tumours; however, there is a lack of methods to validate anti-tumour activity of NK cells in vivo. Herein, we report a new chemiluminescent probe to image in situ the granzyme B-mediated killing activity of NK cells against cancer cells. We have optimised a granzyme B-specific construct using an activatable phenoxydioxetane reporter so that enzymatic cleavage of the probe results in bright chemiluminescence. The probe shows high selectivity for active granzyme B over other proteases and higher signal-to-noise ratios than commercial fluorophores. Finally, we demonstrate that the probe can detect NK cell activity in mouse models, being the first chemiluminescent probe for in vivo imaging of NK cell activity in live tumours.",
keywords = "activatable probes, cancer, chemiluminescence, immunology, natural killer cells",
author = "Scott, {Jamie I.} and Sara Gutkin and Ori Green and Thompson, {Emily J.} and Takanori Kitamura and Doron Shabat and Marc Vendrell",
note = "Publisher Copyright: {\textcopyright} 2021 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH",
year = "2021",
month = mar,
day = "8",
doi = "10.1002/anie.202011429",
language = "אנגלית",
volume = "60",
pages = "5699--5703",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
publisher = "John Wiley and Sons Ltd",
number = "11",
}
