A fluorescence method for estimation of toxemia: Binding capacity of lipoproteins and albumin in plasma

Svetlana M. Altamentova, Nurith Shaklai, Ruth Arav, Yury I. Miller*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The hazard of toxemia, a condition resulting from the spread of toxins by the bloodstream, is regulated by plasma proteins capable of binding with free toxins. As toxin binding results in a reduction of available binding sites, measuring the proteins' binding capacity can be used to estimate toxemia severity. Suggested by this approach, a novel fluorescence method was developed to determine lipoprotein and albumin binding capacities in whole plasma. The method entails two steps: specific binding of N(n-carboxy)phenylimide-4-dimethyl-aminonaphthalic acid with albumin followed by addition of 12-(9-anthroyloxy)stearic acid which, under these conditions, binds mostly with lipoprotein. Reduced fluorescence intensity of the probes in plasma of patients compared to that of healthy donors reflected saturation of binding sites by toxins, thereby estimating toxemia severity. Poor correlation was found between the lipoprotein and albumin binding abilities, suggesting their independent diagnostic values. The simplicity and rapidity of this method are advantageous for its clinical application.

Original languageEnglish
Pages (from-to)133-149
Number of pages17
JournalClinica Chimica Acta
Volume271
Issue number2
DOIs
StatePublished - 23 Mar 1998

Funding

FundersFunder number
Israeli Ministry of Science and Art

    Keywords

    • Albumin
    • Binding capacity
    • Drugs
    • Fluorescent probes
    • Lipoprotein
    • Toxins

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