TY - JOUR
T1 - A dynamic interface between vacuoles and mitochondria in yeast
AU - Elbaz-Alon, Yael
AU - Rosenfeld-Gur, Eden
AU - Shinder, Vera
AU - Futerman, Anthony H.
AU - Geiger, Tamar
AU - Schuldiner, Maya
N1 - Funding Information:
We thank Jodi Nunnari for sharing the MTS-RFP plasmid and Christian Ungermann and Benedikt Westermann for the mBFP plasmid. We thank Oren Elbaz for immense help with the graphic design, Michal Breker and Silvia Chuartzman for technical assistance with the screening system, and Shai Fuchs for technical assistance with the flow cytometer. We thank Oren Schuldiner, Tslil Ast, and Shai Fuchs for critical reading of the manuscript. The electron microscopy studies were conducted at the Irving and Cherna Moskowitz Center for Nano and Bio-Nano Imaging at the Weizmann Institute of Science. This study was funded by a European Research Council Starting Grant (260395). Y.E.-A. was the recipient of the Weizmann Institute Dean’s postdoctoral fellowship. M.S. is an awardee of the EMBO Young Investigator Program, in conjunction with the Israel Ministry of Science, and a recipient of Marie Curie International Reintegration Grant 239224 of the European Union. A.H.F. is the Joseph Meyerhoff Professor of Biochemistry at the Weizmann Institute of Science. T.G. is supported by the Israel Science Foundation.
PY - 2014/7/14
Y1 - 2014/7/14
N2 - Cellular life depends on continuous transport of lipids and small molecules between mitochondria and the endomembrane system. Recently, endoplasmic reticulum-mitochondrial encounter structure(ERMES) was identified as an important yet nonessential contact for such transport. Using a high-contentscreen in yeast, we found a contact site, marked by Vam6/Vps39, between vacuoles (the yeast lysosomal compartment) and mitochondria, named vCLAMP (va. cuo. le and mitochondria patch). vCLAMP is enriched with ion and amino-acid transporters and has a role in lipid relay between the endomembrane system and mitochondria. Critically, we show that mitochondria are dependent on having one of two contact sites, ERMES or vCLAMP. The absence of one causes expansion of the other,and elimination of both is lethal. Identification of vCLAMP adds to our ability to understand the complexity of interorganellar crosstalk.
AB - Cellular life depends on continuous transport of lipids and small molecules between mitochondria and the endomembrane system. Recently, endoplasmic reticulum-mitochondrial encounter structure(ERMES) was identified as an important yet nonessential contact for such transport. Using a high-contentscreen in yeast, we found a contact site, marked by Vam6/Vps39, between vacuoles (the yeast lysosomal compartment) and mitochondria, named vCLAMP (va. cuo. le and mitochondria patch). vCLAMP is enriched with ion and amino-acid transporters and has a role in lipid relay between the endomembrane system and mitochondria. Critically, we show that mitochondria are dependent on having one of two contact sites, ERMES or vCLAMP. The absence of one causes expansion of the other,and elimination of both is lethal. Identification of vCLAMP adds to our ability to understand the complexity of interorganellar crosstalk.
UR - http://www.scopus.com/inward/record.url?scp=84904270185&partnerID=8YFLogxK
U2 - 10.1016/j.devcel.2014.06.007
DO - 10.1016/j.devcel.2014.06.007
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:84904270185
SN - 1534-5807
VL - 30
SP - 95
EP - 102
JO - Developmental Cell
JF - Developmental Cell
IS - 1
ER -