A dual-function phage regulator controls the response of cohabiting phage elements via regulation of the bacterial SOS response

Gil Azulay, Anna Pasechnek, Olga Stadnyuk, Shai Ran-Sapir, Ana Mejia Fleisacher, Ilya Borovok, Nadejda Sigal, Anat A. Herskovits*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Listeria monocytogenes strain 10403S harbors two phage elements in its chromosome; one produces infective virions and the other tailocins. It was previously demonstrated that induction of the two elements is coordinated, as they are regulated by the same anti-repressor. In this study, we identified AriS as another phage regulator that controls the two elements, bearing the capacity to inhibit their lytic induction under SOS conditions. AriS is a two-domain protein that possesses two distinct activities, one regulating the genes of its encoding phage and the other downregulating the bacterial SOS response. While the first activity associates with the AriS N-terminal AntA/AntB domain, the second associates with its C-terminal ANT/KilAC domain. The ANT/KilAC domain is conserved in many AriS-like proteins of listerial and non-listerial prophages, suggesting that temperate phages acquired such dual-function regulators to align their response with the other phage elements that cohabit the genome.

Original languageEnglish
Article number110723
JournalCell Reports
Volume39
Issue number3
DOIs
StatePublished - 19 Apr 2022

Funding

FundersFunder number
Horizon 2020 Framework Programme817842
European Research Council335400
Israel Science FoundationISF 1381/18

    Keywords

    • CP: Microbiology
    • KilAC
    • Listeria monocytogenes
    • SOS response
    • ant
    • bacteriophage
    • lysogeny
    • pathogen
    • phage

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