TY - JOUR
T1 - A double-selective tissue culture system for isolation of wild-type poliovirus from sewage applied in a long-term environmental surveillance
AU - Manor, Y.
AU - Handsher, R.
AU - Halmut, T.
AU - Neuman, M.
AU - Abramovitz, B.
AU - Mates, A.
AU - Mendelson, E.
PY - 1999/4
Y1 - 1999/4
N2 - We describe a simple, cost-efficient, double-selective method for isolation of wild-type poliovirus from sewage samples containing vaccine polioviruses and other enteroviruses, with a detection limit of 18 to 50 PFU per 1 to 2 liters of sewage. By this method we were able to process 1,700 sewage samples collected between 1991 and 1996, from which 10,472 plaques were isolated, 41 of them being identified as wild-type polioviruses.
AB - We describe a simple, cost-efficient, double-selective method for isolation of wild-type poliovirus from sewage samples containing vaccine polioviruses and other enteroviruses, with a detection limit of 18 to 50 PFU per 1 to 2 liters of sewage. By this method we were able to process 1,700 sewage samples collected between 1991 and 1996, from which 10,472 plaques were isolated, 41 of them being identified as wild-type polioviruses.
UR - http://www.scopus.com/inward/record.url?scp=0032957861&partnerID=8YFLogxK
U2 - 10.1128/aem.65.4.1794-1797.1999
DO - 10.1128/aem.65.4.1794-1797.1999
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C2 - 10103285
AN - SCOPUS:0032957861
VL - 65
SP - 1794
EP - 1797
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 4
ER -
