A comparative study of muscle contraction and superprecipitation using trinitrophenylated myosin and active myosin subfragments

Hanna Gadasi; Avraham Oplatka; Raphael Lamed; Andras Muhlrad

doi:10.1016/S0006-291X(74)80230-5

A comparative study of muscle contraction and superprecipitation using trinitrophenylated myosin and active myosin subfragments

Hanna Gadasi^*, Avraham Oplatka, Raphael Lamed, Andras Muhlrad

^*Corresponding author for this work

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

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Abstract

Myosin and heavy meromyosin (HMM) were trinitrophenylated (TNP-ed) to different tents by 2,4,6-trinitro benzene sulfonate. Protein preparations in which only one active te per molecule has been TNP-ed were isolated by using immobilized ATP affinity chromatography columns. Irrigation of "ghost" myofibrils (i.e. myofibrils from which the myosin has been extracted) with either of these "one-headed" species, followed by the addition of ATP, caused contraction. The myosin derivative exhibited also superprecipitation. On the her hand, fully TNP-ed myosin and HMM could not induce contraction of ghosts and the rst did not give superprecipitation. The relationship between superprecipitation and scle contraction is discussed.

Original language	English
Pages (from-to)	913-918
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	58
Issue number	4
DOIs	https://doi.org/10.1016/S0006-291X(74)80230-5
State	Published - 18 Jun 1974
Externally published	Yes

Funding

Funders	Funder number
Muscular Dystrophy Association

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10.1016/S0006-291X(74)80230-5

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title = "A comparative study of muscle contraction and superprecipitation using trinitrophenylated myosin and active myosin subfragments",

abstract = "Myosin and heavy meromyosin (HMM) were trinitrophenylated (TNP-ed) to different tents by 2,4,6-trinitro benzene sulfonate. Protein preparations in which only one active te per molecule has been TNP-ed were isolated by using immobilized ATP affinity chromatography columns. Irrigation of {"}ghost{"} myofibrils (i.e. myofibrils from which the myosin has been extracted) with either of these {"}one-headed{"} species, followed by the addition of ATP, caused contraction. The myosin derivative exhibited also superprecipitation. On the her hand, fully TNP-ed myosin and HMM could not induce contraction of ghosts and the rst did not give superprecipitation. The relationship between superprecipitation and scle contraction is discussed.",

author = "Hanna Gadasi and Avraham Oplatka and Raphael Lamed and Andras Muhlrad",

note = "Funding Information: Supported by grants from the Muscular Dystrophy Associations of America.",

year = "1974",

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doi = "10.1016/S0006-291X(74)80230-5",

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T1 - A comparative study of muscle contraction and superprecipitation using trinitrophenylated myosin and active myosin subfragments

AU - Gadasi, Hanna

AU - Oplatka, Avraham

AU - Lamed, Raphael

AU - Muhlrad, Andras

N1 - Funding Information: Supported by grants from the Muscular Dystrophy Associations of America.

PY - 1974/6/18

Y1 - 1974/6/18

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AB - Myosin and heavy meromyosin (HMM) were trinitrophenylated (TNP-ed) to different tents by 2,4,6-trinitro benzene sulfonate. Protein preparations in which only one active te per molecule has been TNP-ed were isolated by using immobilized ATP affinity chromatography columns. Irrigation of "ghost" myofibrils (i.e. myofibrils from which the myosin has been extracted) with either of these "one-headed" species, followed by the addition of ATP, caused contraction. The myosin derivative exhibited also superprecipitation. On the her hand, fully TNP-ed myosin and HMM could not induce contraction of ghosts and the rst did not give superprecipitation. The relationship between superprecipitation and scle contraction is discussed.

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A comparative study of muscle contraction and superprecipitation using trinitrophenylated myosin and active myosin subfragments

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