A comparative study of muscle contraction and superprecipitation using trinitrophenylated myosin and active myosin subfragments

Hanna Gadasi*, Avraham Oplatka, Raphael Lamed, Andras Muhlrad

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Myosin and heavy meromyosin (HMM) were trinitrophenylated (TNP-ed) to different tents by 2,4,6-trinitro benzene sulfonate. Protein preparations in which only one active te per molecule has been TNP-ed were isolated by using immobilized ATP affinity chromatography columns. Irrigation of "ghost" myofibrils (i.e. myofibrils from which the myosin has been extracted) with either of these "one-headed" species, followed by the addition of ATP, caused contraction. The myosin derivative exhibited also superprecipitation. On the her hand, fully TNP-ed myosin and HMM could not induce contraction of ghosts and the rst did not give superprecipitation. The relationship between superprecipitation and scle contraction is discussed.

Original languageEnglish
Pages (from-to)913-918
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume58
Issue number4
DOIs
StatePublished - 18 Jun 1974
Externally publishedYes

Funding

FundersFunder number
Muscular Dystrophy Association

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