A comparative analysis of total serum miRNA profiles identifies novel signature that is highly indicative of metastatic melanoma: A pilot study

Eyal Greenberg; Michal J. Besser; Eytan Ben-Ami; Ronnie Shapira-Frommer; Orit Itzhaki; Dragoslav Zikich; Daphna Levy; Adva Kubi; Eran Eyal; Amir Onn; Yehudit Cohen; Iris Barshack; Jacob Schachter; Gal Markel

doi:10.3109/1354750X.2013.816777

A comparative analysis of total serum miRNA profiles identifies novel signature that is highly indicative of metastatic melanoma: A pilot study

Eyal Greenberg, Michal J. Besser, Eytan Ben-Ami, Ronnie Shapira-Frommer, Orit Itzhaki, Dragoslav Zikich, Daphna Levy, Adva Kubi, Eran Eyal, Amir Onn, Yehudit Cohen, Iris Barshack, Jacob Schachter, Gal Markel^*

^*Corresponding author for this work

Clinical Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

Context: Quantification of circulating microRNAs (miRNAs) has recently become feasible and reliable, with most efforts focusing on miRNAs overexpressed by cancer cells. Objective: Identification of a characteristic circulating miRNAs profile in melanoma patients. Methods: We conducted a pilot study comprised of unbiased qPCR comparison of serum miRNA profiles between metastatic melanoma patients and healthy donors. Results: Loss of two normal serum-miRNAs, miR-29c and miR-324-3p, is highly indicative of metastatic melanoma. Hierarchical clustering analysis supported the results and clearly distinguished melanoma patients from healthy donors, metastatic colon and renal cancer patients. Discussion and conclusions: This approach is independent of tumor heterogeneity and is expected to have superior biomarker performances.

Original language	English
Pages (from-to)	502-508
Number of pages	7
Journal	Biomarkers
Volume	18
Issue number	6
DOIs	https://doi.org/10.3109/1354750X.2013.816777
State	Published - Sep 2013

Funding

Funders	Funder number
Ella Institute of Melanoma
Flight Attendant Medical Research Institute

Keywords

Biomarkers
circulating microRNAs
diagnostic
melanoma
serum

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3109/1354750X.2013.816777

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Greenberg, E., Besser, M. J., Ben-Ami, E., Shapira-Frommer, R., Itzhaki, O., Zikich, D., Levy, D., Kubi, A., Eyal, E., Onn, A., Cohen, Y., Barshack, I., Schachter, J., & Markel, G. (2013). A comparative analysis of total serum miRNA profiles identifies novel signature that is highly indicative of metastatic melanoma: A pilot study. Biomarkers, 18(6), 502-508. https://doi.org/10.3109/1354750X.2013.816777

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title = "A comparative analysis of total serum miRNA profiles identifies novel signature that is highly indicative of metastatic melanoma: A pilot study",

abstract = "Context: Quantification of circulating microRNAs (miRNAs) has recently become feasible and reliable, with most efforts focusing on miRNAs overexpressed by cancer cells. Objective: Identification of a characteristic circulating miRNAs profile in melanoma patients. Methods: We conducted a pilot study comprised of unbiased qPCR comparison of serum miRNA profiles between metastatic melanoma patients and healthy donors. Results: Loss of two normal serum-miRNAs, miR-29c and miR-324-3p, is highly indicative of metastatic melanoma. Hierarchical clustering analysis supported the results and clearly distinguished melanoma patients from healthy donors, metastatic colon and renal cancer patients. Discussion and conclusions: This approach is independent of tumor heterogeneity and is expected to have superior biomarker performances.",

keywords = "Biomarkers, circulating microRNAs, diagnostic, melanoma, serum",

author = "Eyal Greenberg and Besser, {Michal J.} and Eytan Ben-Ami and Ronnie Shapira-Frommer and Orit Itzhaki and Dragoslav Zikich and Daphna Levy and Adva Kubi and Eran Eyal and Amir Onn and Yehudit Cohen and Iris Barshack and Jacob Schachter and Gal Markel",

note = "Funding Information: This work was supported by the Ella Institute of Melanoma and the Flight Attendants Medical Research Institute (FAMRI).",

year = "2013",

month = sep,

doi = "10.3109/1354750X.2013.816777",

language = "אנגלית",

volume = "18",

pages = "502--508",

journal = "Biomarkers",

issn = "1354-750X",

publisher = "Informa Healthcare",

number = "6",

}

Greenberg, E, Besser, MJ, Ben-Ami, E, Shapira-Frommer, R, Itzhaki, O, Zikich, D, Levy, D, Kubi, A, Eyal, E, Onn, A, Cohen, Y, Barshack, I , Schachter, J & Markel, G 2013, 'A comparative analysis of total serum miRNA profiles identifies novel signature that is highly indicative of metastatic melanoma: A pilot study', Biomarkers, vol. 18, no. 6, pp. 502-508. https://doi.org/10.3109/1354750X.2013.816777

TY - JOUR

T1 - A comparative analysis of total serum miRNA profiles identifies novel signature that is highly indicative of metastatic melanoma

T2 - A pilot study

AU - Greenberg, Eyal

AU - Besser, Michal J.

AU - Ben-Ami, Eytan

AU - Shapira-Frommer, Ronnie

AU - Itzhaki, Orit

AU - Zikich, Dragoslav

AU - Levy, Daphna

AU - Kubi, Adva

AU - Eyal, Eran

AU - Onn, Amir

AU - Cohen, Yehudit

AU - Barshack, Iris

AU - Schachter, Jacob

AU - Markel, Gal

N1 - Funding Information: This work was supported by the Ella Institute of Melanoma and the Flight Attendants Medical Research Institute (FAMRI).

PY - 2013/9

Y1 - 2013/9

N2 - Context: Quantification of circulating microRNAs (miRNAs) has recently become feasible and reliable, with most efforts focusing on miRNAs overexpressed by cancer cells. Objective: Identification of a characteristic circulating miRNAs profile in melanoma patients. Methods: We conducted a pilot study comprised of unbiased qPCR comparison of serum miRNA profiles between metastatic melanoma patients and healthy donors. Results: Loss of two normal serum-miRNAs, miR-29c and miR-324-3p, is highly indicative of metastatic melanoma. Hierarchical clustering analysis supported the results and clearly distinguished melanoma patients from healthy donors, metastatic colon and renal cancer patients. Discussion and conclusions: This approach is independent of tumor heterogeneity and is expected to have superior biomarker performances.

AB - Context: Quantification of circulating microRNAs (miRNAs) has recently become feasible and reliable, with most efforts focusing on miRNAs overexpressed by cancer cells. Objective: Identification of a characteristic circulating miRNAs profile in melanoma patients. Methods: We conducted a pilot study comprised of unbiased qPCR comparison of serum miRNA profiles between metastatic melanoma patients and healthy donors. Results: Loss of two normal serum-miRNAs, miR-29c and miR-324-3p, is highly indicative of metastatic melanoma. Hierarchical clustering analysis supported the results and clearly distinguished melanoma patients from healthy donors, metastatic colon and renal cancer patients. Discussion and conclusions: This approach is independent of tumor heterogeneity and is expected to have superior biomarker performances.

KW - Biomarkers

KW - circulating microRNAs

KW - diagnostic

KW - melanoma

KW - serum

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A comparative analysis of total serum miRNA profiles identifies novel signature that is highly indicative of metastatic melanoma: A pilot study

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Keywords

UN SDGs

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