A comparative analysis of total serum miRNA profiles identifies novel signature that is highly indicative of metastatic melanoma: A pilot study

Eyal Greenberg, Michal J. Besser, Eytan Ben-Ami, Ronnie Shapira-Frommer, Orit Itzhaki, Dragoslav Zikich, Daphna Levy, Adva Kubi, Eran Eyal, Amir Onn, Yehudit Cohen, Iris Barshack, Jacob Schachter, Gal Markel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Context: Quantification of circulating microRNAs (miRNAs) has recently become feasible and reliable, with most efforts focusing on miRNAs overexpressed by cancer cells. Objective: Identification of a characteristic circulating miRNAs profile in melanoma patients. Methods: We conducted a pilot study comprised of unbiased qPCR comparison of serum miRNA profiles between metastatic melanoma patients and healthy donors. Results: Loss of two normal serum-miRNAs, miR-29c and miR-324-3p, is highly indicative of metastatic melanoma. Hierarchical clustering analysis supported the results and clearly distinguished melanoma patients from healthy donors, metastatic colon and renal cancer patients. Discussion and conclusions: This approach is independent of tumor heterogeneity and is expected to have superior biomarker performances.

Original languageEnglish
Pages (from-to)502-508
Number of pages7
JournalBiomarkers
Volume18
Issue number6
DOIs
StatePublished - Sep 2013

Funding

FundersFunder number
Ella Institute of Melanoma
Flight Attendant Medical Research Institute

    Keywords

    • Biomarkers
    • circulating microRNAs
    • diagnostic
    • melanoma
    • serum

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