A Combined Procedure For Preparation of Plastocyanin, Ferredoxin and Cf1

C. F. Yocum, N. Nelson, E. Racker

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14 Scopus citations

Abstract

Homogeneous preparations of ferredoxin, plastocyanin, and chloroplast coupling factor (CF1) have been isolated from spinach by a combined procedure in which supernatants from preparation of chloroplasts are used for isolation of ferredoxin and the chloroplasts serve as the source of plastocyanin. The proteins were purified by DEAE-cellulose chromatography and gel filtration, after precipitation with acetone in the case of ferredoxin or release from membranes in the case of plastocyanin. The proteins obtained by this procedure are pure, as evidenced by absorption ratios (ferredoxin, A420/A276 = 0.47-0.48; plastocyanin, A278/A597 = 1.2) and by the fact that both proteins migrate as single bands on polyacrylamide gels in the presence of sodium dodecyl sulfate.

Original languageEnglish
Pages (from-to)305-317
Number of pages13
JournalPreparative Biochemistry
Volume5
Issue number4
DOIs
StatePublished - 1 Jan 1975
Externally publishedYes

Funding

FundersFunder number
National Science FoundationGBX-30850X, GM51584-01
National Institutes of Health

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