A cell line with unusual characteristics from an ovarian carcinoma patient: Modulation of sensitivity to antitumour drugs

Lina Wasserman, Rami Aviram, Hanoch Levavi, Jardena Ovadia, Yona Shneyuor, Amos Frisch, Orit Blau, Einat Beery, Abraham Novogrodsky, Jardena Nordenberg

Research output: Contribution to journalArticlepeer-review

Abstract

A cell line (GZL-8) was established by cloning from ascitic fluid of an untreated ovarian carcinoma patient. The cells grew rapidly, accumulated lipids and showed chromosomal alterations. One of the marker chromosomes showed characteristics of a Y-like chromosome. This unusual finding was confirmed by DNA hybridisation using specific probes to the Y chromosome. The cells stained with fluorescent antibodies to desmoplakin and cytokeratins 8, 18, 19, and weakly with vimentin but not with desmin. The presence of epithelial membrane antigen, human milk fat globulin, α-lactalbumin, α-fetoprotein, placental alkaline phosphatase and oestrogen receptor-related antigen was demonstrated by indirect immunoperoxidase staining, but no CA-125 antigen could be detected. The cells showed positive reaction with antibodies to P-glycoprotein. The function of the P-glycoprotein transport system was demonstrated by the rhodamine-123 release test. The cells were initially responsive to doxorubicin, and to high concentrations of cisplatin. Growth inhibition by doxorubicin, especially at low doses was enhanced by the addition of verapamil or tamoxifen. This was shown by the soft agar clonogenic assay, by direct cell counting and by the MTT reducing test. Our results show that combination between drug and sensitivity modulators may be of potential clinical value in ovarian cancer.

Original languageEnglish
Pages (from-to)22-27
Number of pages6
JournalEuropean Journal of Cancer
Volume28
Issue number1
DOIs
StatePublished - Jan 1992

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