A cationic hydroxysuccinimide ester A reagent for labeling exterior membrane proteins

Nava Zisapel*, Uriel Z. Littauer

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


3H-labeled N,N,N,trimethylamino-β-alanyl-N-hydroxysuccinimido ester ([3H]TMAS), a new cationic membrane reagent, was synthesized. TMAS was shown to be impermeant through human erythrocyte membranes. Under mild physiological conditions TMAS reacted primarily with amino groups of the membrane proteins and lipids. The pattern of erythrocyte proteins labeled with [3H]TMAS was examined by polyacrylamide gel electrophoresis under denaturing conditions. Externally oriented labeling of intact erythrocytes revealed a major radioactive protein with an apparent molecular weight of 90 000. By labeling ghost preparations with [3H]TMAS the radioactivity incorporated into all the major Coomassie Brilliant Blue bands resolved by gel electrophoresis. The agreement between the results obtained with anionic and cationic amino reactive probes indicates that the ionic character of the reagent has a minor effect on the pattern of labeled exterior polypeptides observed in erythrocytes.

Original languageEnglish
Pages (from-to)156-162
Number of pages7
JournalBBA - Biomembranes
Issue number1
StatePublished - 11 Sep 1978
Externally publishedYes


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