A Ca²⁺/CaM-regulated transcriptional switch modulates stomatal development in response to water deficit

Calcium (Ca²⁺) signals are decoded by the Ca²⁺-sensor protein calmodulin (CaM) and are transduced to Ca²⁺/CaM-binding transcription factors to directly regulate gene expression necessary for acclimation responses in plants. The molecular mechanisms of Ca²⁺/CaM signal transduction processes and their functional significance remains enigmatic. Here we report a novel Ca²⁺/CaM signal transduction mechanism that allosterically regulates DNA-binding activity of GT2-LIKE 1 (GTL1), a transrepressor of STOMATAL DENSITY AND DISTRIBUTION 1 (SDD1), to repress stomatal development in response to water stress. We demonstrated that Ca²⁺/CaM interaction with the 2^nd helix of the GTL1 N-terminal trihelix DNA-binding domain (GTL1N) destabilizes a hydrophobic core of GTL1N and allosterically inhibits 3^rd helix docking to the SDD1 promoter, leading to osmotic stress-induced Ca²⁺/CaM-dependent activation (de-repression) of SDD1 expression. This resulted in GTL1-dependent repression of stomatal development in response to water-deficit stress. Together, our results demonstrate that a Ca²⁺/CaM-regulated transcriptional switch on a trihelix transrepressor directly transduces osmotic stress to repress stomatal development to improve plant water-use efficiency as an acclimation response.