@article{b5744ae8c50d46efb6121d2ca577662d,
title = "A Ca2+/CaM-regulated transcriptional switch modulates stomatal development in response to water deficit",
abstract = "Calcium (Ca2+) signals are decoded by the Ca2+-sensor protein calmodulin (CaM) and are transduced to Ca2+/CaM-binding transcription factors to directly regulate gene expression necessary for acclimation responses in plants. The molecular mechanisms of Ca2+/CaM signal transduction processes and their functional significance remains enigmatic. Here we report a novel Ca2+/CaM signal transduction mechanism that allosterically regulates DNA-binding activity of GT2-LIKE 1 (GTL1), a transrepressor of STOMATAL DENSITY AND DISTRIBUTION 1 (SDD1), to repress stomatal development in response to water stress. We demonstrated that Ca2+/CaM interaction with the 2nd helix of the GTL1 N-terminal trihelix DNA-binding domain (GTL1N) destabilizes a hydrophobic core of GTL1N and allosterically inhibits 3rd helix docking to the SDD1 promoter, leading to osmotic stress-induced Ca2+/CaM-dependent activation (de-repression) of SDD1 expression. This resulted in GTL1-dependent repression of stomatal development in response to water-deficit stress. Together, our results demonstrate that a Ca2+/CaM-regulated transcriptional switch on a trihelix transrepressor directly transduces osmotic stress to repress stomatal development to improve plant water-use efficiency as an acclimation response.",
author = "Yoo, {Chan Yul} and Noel Mano and Aliza Finkler and Hua Weng and Day, {Irene S.} and Reddy, {Anireddy S.N.} and Poovaiah, {B. W.} and Hillel Fromm and Hasegawa, {Paul M.} and Mickelbart, {Michael V.}",
note = "Publisher Copyright: {\textcopyright} 2019, The Author(s).",
year = "2019",
month = dec,
day = "1",
doi = "10.1038/s41598-019-47529-2",
language = "אנגלית",
volume = "9",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Research",
number = "1",
}