TY - JOUR
T1 - A biophysical perspective on the cellulosome
T2 - new opportunities for biomass conversion
AU - Ding, Shi You
AU - Xu, Qi
AU - Crowley, Michael
AU - Zeng, Yining
AU - Nimlos, Mark
AU - Lamed, Raphael
AU - Bayer, Edward A.
AU - Himmel, Michael E.
N1 - Funding Information:
This work was supported partly by the DOE Office of Biological and Environmental Research-Genomes to Life Program through the BioEnergy Science Center (BESC). This work was also supported by the Israel Science Foundation (Grant Nos 422/05 and 159/07), and by grants from the United States–Israel Binational Science Foundation (BSF), Jerusalem, Israel. The authors also wish to thank Lee Lynd for helpful comments made during the writing of this work.
PY - 2008/6
Y1 - 2008/6
N2 - The cellulosome is a multiprotein complex, produced primarily by anaerobic microorganisms, which functions to degrade lignocellulosic materials. An important topic of current debate is whether cellulosomal systems display greater ability to deconstruct complex biomass materials (e.g. plant cell walls) than nonaggregated enzymes, and in so doing would be appropriate for improved, commercial bioconversion processes. To sufficiently understand the complex macromolecular processes between plant cell wall polymers, cellulolytic microbes, and their secreted enzymes, a highly concerted research approach is required. Adaptation of existing biophysical techniques and development of new science tools must be applied to this system. This review focuses on strategies likely to permit improved understanding of the bacterial cellulosome using biophysical approaches, with emphasis on advanced imaging and computational techniques.
AB - The cellulosome is a multiprotein complex, produced primarily by anaerobic microorganisms, which functions to degrade lignocellulosic materials. An important topic of current debate is whether cellulosomal systems display greater ability to deconstruct complex biomass materials (e.g. plant cell walls) than nonaggregated enzymes, and in so doing would be appropriate for improved, commercial bioconversion processes. To sufficiently understand the complex macromolecular processes between plant cell wall polymers, cellulolytic microbes, and their secreted enzymes, a highly concerted research approach is required. Adaptation of existing biophysical techniques and development of new science tools must be applied to this system. This review focuses on strategies likely to permit improved understanding of the bacterial cellulosome using biophysical approaches, with emphasis on advanced imaging and computational techniques.
UR - http://www.scopus.com/inward/record.url?scp=46549089294&partnerID=8YFLogxK
U2 - 10.1016/j.copbio.2008.04.008
DO - 10.1016/j.copbio.2008.04.008
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AN - SCOPUS:46549089294
SN - 0958-1669
VL - 19
SP - 218
EP - 227
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
IS - 3
ER -