A bioautographic method for identification of choline and its derivatives

Lawrence M. Lewin, Nathan Marcus

Research output: Contribution to journalArticlepeer-review

Abstract

A sensitive and specific bioautographic method for detecting choline and its derivatives has been developed, utilizing a Neurospora crassa mutant. As little as 0.03 μg of choline chloride is detectable. The applicability of the method for detection of choline derivatives in lipid hydrolyzates is discussed. A method for determining the rate of diffusion of compounds from paper chromatograms into bioautograph agar is described.

Original languageEnglish
Pages (from-to)96-100
Number of pages5
JournalAnalytical Biochemistry
Volume10
Issue number1
DOIs
StatePublished - Jan 1965

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