A Binary Cre Transgenic Approach Dissects Microglia and CNS Border-Associated Macrophages

Jung Seok Kim; Masha Kolesnikov; Shany Peled-Hajaj; Isabelle Scheyltjens; Yuan Xia; Sebastien Trzebanski; Zhana Haimon; Anat Shemer; Alisa Lubart; Hannah Van Hove; Louise Chappell-Maor; Sigalit Boura-Halfon; Kiavash Movahedi; Pablo Blinder; Steffen Jung

doi:10.1016/j.immuni.2020.11.007

A Binary Cre Transgenic Approach Dissects Microglia and CNS Border-Associated Macrophages

Jung Seok Kim, Masha Kolesnikov, Shany Peled-Hajaj, Isabelle Scheyltjens, Yuan Xia, Sebastien Trzebanski, Zhana Haimon, Anat Shemer, Alisa Lubart, Hannah Van Hove, Louise Chappell-Maor, Sigalit Boura-Halfon, Kiavash Movahedi, Pablo Blinder, Steffen Jung^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

110 Scopus citations

Abstract

The brain harbors both parenchymal microglia and perivascular macrophages, individual functions of which remain incompletely understood. Kim et al. establish a binary transgenic split Cre system that allows differential targeting and translatome analysis of these cells in the mouse.

Original language	English
Pages (from-to)	176-190.e7
Journal	Immunity
Volume	54
Issue number	1
DOIs	https://doi.org/10.1016/j.immuni.2020.11.007
State	Published - 12 Jan 2021

Funding

Funders	Funder number
Blythe Brenden-Mann Foundation
Roland N. Karlen Foundation
American Brain Foundation
European Research Council	ERC 340345
Minerva Foundation
Deutsche Forschungsgemeinschaft	CRC/TRR167
Fonds Wetenschappelijk Onderzoek
Israel Science Foundation	887/11
Innoviris	BB2B 2015-2

Keywords

Lyve1 BAM
RiboTag
binary transgenic
brain macrophages
intersectional genetics
meninges
microglia
perivascular macrophages
pia mater
split cre

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10.1016/j.immuni.2020.11.007

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Kim, J. S., Kolesnikov, M., Peled-Hajaj, S., Scheyltjens, I., Xia, Y., Trzebanski, S., Haimon, Z., Shemer, A., Lubart, A., Van Hove, H., Chappell-Maor, L., Boura-Halfon, S., Movahedi, K., Blinder, P., & Jung, S. (2021). A Binary Cre Transgenic Approach Dissects Microglia and CNS Border-Associated Macrophages. Immunity, 54(1), 176-190.e7. https://doi.org/10.1016/j.immuni.2020.11.007

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Kim, JS, Kolesnikov, M, Peled-Hajaj, S, Scheyltjens, I, Xia, Y, Trzebanski, S, Haimon, Z, Shemer, A, Lubart, A, Van Hove, H, Chappell-Maor, L, Boura-Halfon, S, Movahedi, K, Blinder, P & Jung, S 2021, 'A Binary Cre Transgenic Approach Dissects Microglia and CNS Border-Associated Macrophages', Immunity, vol. 54, no. 1, pp. 176-190.e7. https://doi.org/10.1016/j.immuni.2020.11.007

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AU - Xia, Yuan

AU - Trzebanski, Sebastien

AU - Haimon, Zhana

AU - Shemer, Anat

AU - Lubart, Alisa

AU - Van Hove, Hannah

AU - Chappell-Maor, Louise

AU - Boura-Halfon, Sigalit

AU - Movahedi, Kiavash

AU - Blinder, Pablo

AU - Jung, Steffen

PY - 2021/1/12

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KW - intersectional genetics

KW - meninges

KW - microglia

KW - perivascular macrophages

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A Binary Cre Transgenic Approach Dissects Microglia and CNS Border-Associated Macrophages

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