[55] Immobilized Nucleotides for Affinity Chromatography

Meir Wilchek, Raphael Lamed

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Nucleosides and nucleotide phosphates occur in many biological systems and act as enzyme substrates, as cofactors, and as coenzymes. Thus, covalently binding these compounds to insoluble matrices provides a useful tool for the study of their interactions with proteins as well as media for affinity chromatography. A simple and general method for coupling nucleosides, nucleotide phosphate, and coenzymes possessing vicinal free hydroxyl groups to agarose hydrazide columns, has been described in this chapter.

Original languageEnglish
Pages (from-to)475-479
Number of pages5
JournalMethods in Enzymology
Volume34
Issue numberC
DOIs
StatePublished - 1 Jan 1974

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