TY - JOUR
T1 - [55] Immobilized Nucleotides for Affinity Chromatography
AU - Wilchek, Meir
AU - Lamed, Raphael
PY - 1974/1/1
Y1 - 1974/1/1
N2 - Nucleosides and nucleotide phosphates occur in many biological systems and act as enzyme substrates, as cofactors, and as coenzymes. Thus, covalently binding these compounds to insoluble matrices provides a useful tool for the study of their interactions with proteins as well as media for affinity chromatography. A simple and general method for coupling nucleosides, nucleotide phosphate, and coenzymes possessing vicinal free hydroxyl groups to agarose hydrazide columns, has been described in this chapter.
AB - Nucleosides and nucleotide phosphates occur in many biological systems and act as enzyme substrates, as cofactors, and as coenzymes. Thus, covalently binding these compounds to insoluble matrices provides a useful tool for the study of their interactions with proteins as well as media for affinity chromatography. A simple and general method for coupling nucleosides, nucleotide phosphate, and coenzymes possessing vicinal free hydroxyl groups to agarose hydrazide columns, has been described in this chapter.
UR - http://www.scopus.com/inward/record.url?scp=0016145163&partnerID=8YFLogxK
U2 - 10.1016/S0076-6879(74)34058-X
DO - 10.1016/S0076-6879(74)34058-X
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C2 - 4155785
AN - SCOPUS:0016145163
SN - 0076-6879
VL - 34
SP - 475
EP - 479
JO - Methods in Enzymology
JF - Methods in Enzymology
IS - C
ER -