5'-Terminal sequences and coding region of late simian virus 40 mRNAs are derived from noncontiguous segments of the viral genome.

S. Lavi*, Y. Groner

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The region of the simian virus 40 genome complementary to the 5' end of the most abundant poly(A)-containing 19S and 16S mRNAs was mapped by hybridization of double-labeled RNA ([3H]methyl group and [14C]uridine) to specific DNA fragments. Chemical identification of methylated residues indicated that a common "leader" sequence adjacent to the 5' terminus of both 19S and 16S mRNA is transcribed from DNA sequences located between 0.67 and 0.76 map units. The estimated size of this "leader" RNA, which does not code for any known viral protein, is 170-200 nucleotides. Our results indicate that sequences complementary to the "leader" region and coding portion of 16S mRNA are located in separate parts of the simian virus 40 genome.

Original languageEnglish
Pages (from-to)5323-5327
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume74
Issue number12
DOIs
StatePublished - Dec 1977

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