TY - JOUR
T1 - 5-HT and cAMP induce the formation of coated pits and vesicles and increase the expression of clathrin light chain in sensory neurons of aplysia
AU - Hu, Yinghe
AU - Barzilai, Ari
AU - Chen, Mary
AU - Bailey, Craig H.
AU - Kandel, Eric R.
N1 - Funding Information:
We thank Frances Brodsky for her advice during the course of this work and for her suggestions on this manuscript, Mark May-ford for providing the cDNA library, Sharon Foster for technical support, Dietmar Kuhl fortheHSC7Oprobeand his useful discussions, Sarah Mack and Charles Lam for preparing the figures, and Andy Krawetz and Harriet Ayers for typing the manuscript. This work was supported in part by the Howard Hughes Medical Institute to E. R. K. and National Institutes of Health grants MH37134 and GM32099 to C. H. B. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC Section 1734 solely to indicate this fact.
PY - 1993/5
Y1 - 1993/5
N2 - In the course of studying proteins involved in long-term facilitation in Aplysia, we found that 5-HT and cAMP, a second messenger activated by 5-HT, lead to the removal of a set of N-CAM-related cell adhesion molecules (apCAMs) from the surface membrane of sensory neurons by means of receptor-mediated endocytosis. Here we describe that, as part of this coordinated program for endocytosis, 5-HT and CAMP also induce in the sensory neurons an increase in the density of coated pits and coated vesicles and an increase in the expression of the light chain of Aplysia clathrin (apClathrin). The clathrin-related endocytosis seems designed to internalize and redistribute apCAMs and other surface membrane proteins in the sensory neurons, and thus it appears to constitute one of the initial steps in the growth of new synaptic connections that accompanies long-term facilitation.
AB - In the course of studying proteins involved in long-term facilitation in Aplysia, we found that 5-HT and cAMP, a second messenger activated by 5-HT, lead to the removal of a set of N-CAM-related cell adhesion molecules (apCAMs) from the surface membrane of sensory neurons by means of receptor-mediated endocytosis. Here we describe that, as part of this coordinated program for endocytosis, 5-HT and CAMP also induce in the sensory neurons an increase in the density of coated pits and coated vesicles and an increase in the expression of the light chain of Aplysia clathrin (apClathrin). The clathrin-related endocytosis seems designed to internalize and redistribute apCAMs and other surface membrane proteins in the sensory neurons, and thus it appears to constitute one of the initial steps in the growth of new synaptic connections that accompanies long-term facilitation.
UR - http://www.scopus.com/inward/record.url?scp=0027233570&partnerID=8YFLogxK
U2 - 10.1016/0896-6273(93)90207-8
DO - 10.1016/0896-6273(93)90207-8
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AN - SCOPUS:0027233570
VL - 10
SP - 921
EP - 929
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 5
ER -