[45] Extracellular Aminopeptidase from Clostridium histolyticum

Efrat Kessler, Arieh Yaron

Research output: Contribution to journalArticlepeer-review

Abstract

Clostridial aminopeptidase (CAP) is an extracellular enzyme of broad substrate specificity that has been isolated from the culture filtrate of Clostridium histolyticum and purified to homogeneity. This chapter describes the procedure followed based on the assay principle: The enzyme cleaves efficiently various N-terminal amino acid residues, including proline, from peptides of low and of high molecular weight. The tripeptide Pro-Gly-Pro, which is resistant to most proteolytic enzymes, is used as a selective substrate for the routine assay during the purification of CAP. Proline and Gly-Pro are produced as the only products and the amount of proline formed is determined colorimetrically by the acid ninhydrin method. It describes the purification procedure starting from Cultivation of Bacteria and Preparation of the Culture Filtrate. It also explains the key properties of the aminopeptidase, including its physical and kinetic properties and its stability.

Original languageEnglish
Pages (from-to)544-552
Number of pages9
JournalMethods in Enzymology
Volume45
Issue numberC
DOIs
StatePublished - 1 Jan 1976

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