3D-Printed Hydrodynamic Focusing Lab-on-a-Chip Device for Impedance Flow Particle Analysis

Dayananda Desagani, Shani Kleiman, Teddy Zagardan, Hadar Ben-Yoav*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Particles analysis, such as cell counting and differentiation, are widely used for the diagnosis and monitoring of several medical conditions, such as during inflammation. Three-dimensional-printed lab-on-a-chip (LOC) devices, which can utilize one of the cell counting methods, can bring this technology to remote locations through its cost-efficient advantages and easy handling. We present a three-dimensional-printed LOC device with integrated electrodes. To overcome the limited resolution of a 3D printer, we utilized a flow-focusing design. We modeled and simulated the mass transfer and flow dynamics in the LOC by incorporating a flow-focusing design and reached an optimal channel diameter of 0.5 mm, resulting in a flow-focusing distance of <60 µm. We also used electrochemical impedance spectroscopy to enable the dependence of the electrode–solution interface on the flow-focusing properties. Finally, we highlighted the proof-of-concept detection of microspheres (6 µm diameter), which model biological cells that flow in the channel, by recording the electrochemical impedance at 10 kHz, thus showing the potential of a future point-of-care (POC) device.

Original languageEnglish
Article number283
JournalChemosensors
Volume11
Issue number5
DOIs
StatePublished - May 2023
Externally publishedYes

Keywords

  • 3D printing
  • electrochemical impedance spectroscopy
  • finite element method modeling
  • flow cytometry
  • flow focusing
  • fused filament fabrication
  • lab-on-a-chip
  • microfluidics
  • point-of-care detection
  • rapid prototyping

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