Affinity cytochemistry

Meir Wilchek, Edward A. Bayer, Ehud Skutelsky

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The use of avidin–biotin technology has progressed rapidly in a variety of areas, such as immunoassay and gene probes; the broadest application remains in the localization of target molecules, both on cell surfaces and in the intracellular matrix. Because localization usually involves a cell or tissue which can be considered a solid-phase system, the application of avidin–biotin technology to other fields was clearly an outgrowth of the initial development of the technique for localization investigations. Avidin–biotin technology can be applied for direct localization and binder-mediated visualization of cell-associated components. Using a combination of neuraminidase and galactose oxidase, it is possible to localize the penultimate galactose and to measure the distance of the sialic acid from the cell membrane. The binder-mediated approach is of much broader application and is the most widely accepted approach for localization.

Original languageEnglish
Pages (from-to)340-342
Number of pages3
JournalMethods in Enzymology
Volume184
Issue numberC
DOIs
StatePublished - 1990

Funding

FundersFunder number
Officeo f Naval Research
National Institutes of Health
U.S. Department of Health and Human ServicesNO1-CO-74101
National Cancer Institute

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