TY - JOUR
T1 - Affinity cytochemistry
AU - Wilchek, Meir
AU - Bayer, Edward A.
AU - Skutelsky, Ehud
N1 - Funding Information:
The authors thank Dr. Sharon Moore for helpful discussion and critical review of the manuscript, Candice Harris and Greg Beal for technical assistance, and Julie Ratlifff or preparation of the manuscript. Research was sponsored in part by the National Cancer Institute, U.S. Department of Health and Human Services, under Contract NO1-CO-74101 with BRI (R.F.), and grants from the National Institutes of Health and the Officeo f Naval Research (A.R.).
PY - 1990
Y1 - 1990
N2 - The use of avidin–biotin technology has progressed rapidly in a variety of areas, such as immunoassay and gene probes; the broadest application remains in the localization of target molecules, both on cell surfaces and in the intracellular matrix. Because localization usually involves a cell or tissue which can be considered a solid-phase system, the application of avidin–biotin technology to other fields was clearly an outgrowth of the initial development of the technique for localization investigations. Avidin–biotin technology can be applied for direct localization and binder-mediated visualization of cell-associated components. Using a combination of neuraminidase and galactose oxidase, it is possible to localize the penultimate galactose and to measure the distance of the sialic acid from the cell membrane. The binder-mediated approach is of much broader application and is the most widely accepted approach for localization.
AB - The use of avidin–biotin technology has progressed rapidly in a variety of areas, such as immunoassay and gene probes; the broadest application remains in the localization of target molecules, both on cell surfaces and in the intracellular matrix. Because localization usually involves a cell or tissue which can be considered a solid-phase system, the application of avidin–biotin technology to other fields was clearly an outgrowth of the initial development of the technique for localization investigations. Avidin–biotin technology can be applied for direct localization and binder-mediated visualization of cell-associated components. Using a combination of neuraminidase and galactose oxidase, it is possible to localize the penultimate galactose and to measure the distance of the sialic acid from the cell membrane. The binder-mediated approach is of much broader application and is the most widely accepted approach for localization.
UR - http://www.scopus.com/inward/record.url?scp=0025285059&partnerID=8YFLogxK
U2 - 10.1016/0076-6879(90)84290-W
DO - 10.1016/0076-6879(90)84290-W
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AN - SCOPUS:0025285059
SN - 0076-6879
VL - 184
SP - 340
EP - 342
JO - Methods in Enzymology
JF - Methods in Enzymology
IS - C
ER -