2-Nitroimipramine: A selective irreversible inhibitor of [3H] serotonin uptake and [3H] imipramine binding in platelets

Moshe Rehavi*, Yitzhak Ittah, Kenner C. Rice, Phil Skolnick, Frederick K. Goodwin, Steven M. Paul

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The effect(s) of a new imipramine analogue, 2-nitroimipramine, on high affinity [3H] imipramine binding and [3H] serotonin uptake in human platelets were studied. 2-Nitroimipramine was found not only to be a very potent inhibitor of [3H] imipramine binding and [3H] serotonin uptake but was found to irreversibly inhibit binding and uptake simultaneously. This finding supports previous observations from our laboratory and others that high affinity imipramine binding labels serotonin uptake or transport sites. 2-Nitroimipramine should prove an important tool for subsequent studies of the molecular mechanism(s) involved in the transport of serotonin and the binding of imipramine to platelet and brain membranes.

Original languageEnglish
Pages (from-to)954-959
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume99
Issue number3
DOIs
StatePublished - 15 Apr 1981
Externally publishedYes

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